Question: A 28-year old bride-to-be is getting married the first week of B) New nucleotides are positioned in the proper order by DNA polymerase. Question: What is the total magnification of each power objective on the Which of the following is not a true statement comparing prokaryotic and eukaryotic DNA replication? The lagging strand of DNA is started by an RNA primer. 86) The four bases that make up the DNA “ladder” are: ___. a. 80) If you start a PCR amplification with one molecule of double stranded DNA, how many. c. It is associated with a sliding clamp only on the leading strand. Producing multiple copies of a recombinant DNA by transforming bacteria. C. A new DNA strand is synthesized with the same polarity (5' and 3' ends) as the template strand. Is defined as a mix of human and animal DNA. Which of the following statements is accurate for the PCR – polymerase chain reaction? d. Its exonuclease activity is involved in proofreading. Which of the following is not true of the polymerase chain reaction (PCR)? DNA replication proceeds in only one direction around the bacterial chromosome. O It uses ATP hydrolysis to fuel polymerization. on each plate to each other? d) a) anneal the primers; b) denature the DNA strand; c) activate the polymerase enzyme. Which of the following statements about DNA replication is NOT true? © 2003-2020 Chegg Inc. All rights reserved. o It needs a template DNA strand. The company also reserves no rights to use or reproduce any content after the client has paid for it. cleavage by restriction enzymes of host bacterium? DNA (deoxyribonucleic acid) polymerase is an enzyme that The molecules that. Which of the following is not true about Taq DNA polymerase? A. PCR requires DNA primers to initiate synthesis of a strand complimentary in sequence to the template. law of complementary bases during the addition of new Which of the following statements about the polymerase chain reaction are true. c. DNA replication proceeds in only one direction around the bacterial chromosome d. Multiple replication forks are possible on a bacterial chromosome. Is defined as a mix of human and animal DNA. b. b) a) denature the DNA sample; b) allow the primers to bind the templates; c) extend each strand. It follows the c. During transcription of a gene, RNA polymerase reads only one strand of DNA. Explanation: DNA polymerase is an enzyme which is used to make new DNA. O It uses ATP hydrolysis to fuel polymerization. Which of the following is required to perform polymerase chain reaction ? Terms None of the above (all are required) Which IS NOT true about complimentary base-pairing: a. chemical filtration ,. b. RNA polymerase binds to a promoter to initiate transcription. Is defined as a mix of human and animal DNA. Match following enzyme with its activity; 1) Forms phophodiester bond; 2) Adds phosphate group; 3) Remove Phosphate group; 4) adds, 25. O It uses ATP hydrolysis to fuel polymerization. She wants to wear …. During DNA replication, DNA polymerase adds nucleotides to form a new DNA strand using the original DNA strand as a template. Which me…, Question: Complete the following paragraph to explain the relationship between nicotinic and muscarinic cho…, Question: Why wouldn’t it be advisable to compare growth of the organisms c. Is created from embryonic stem cells. a. RNA polymerase adds a ribonucleotide to the 3’ end of a growing RNA molecule. (a) Automated PCR machines are called thermal cyclers (b) A thermostable DNA polymerase is required (c) Millions to billions of desired DNA copies can be produced from microgram quantities of DNA (d) All of the above. The DNA polymerase is an enzyme that synthesises DNA in all living organism and thus present in almost all organism on earth. replication, DNA polymerase adds nucleotides to form a new DNA b. * 20. Privacy View desktop site, Solution) 1) option C is answer. Ther…. 19. Which of the following is NOT true of the polymerase chain reaction (PCR)? c. Producing a transgenic animal by inserting recombinant DNA into an embryo. b. It is important for transcription c. It is important for translation d. It is important for PCR e. None of the above (all of the statements are true) Recombinant DNA: a. This DNA polymerase enzymatically assembles a new DNA strand from DNA building blocks, the nucleotides, by using single-stranded DNA as a template and DNA oligonucleotides, which are required for initiation of DNA synthesis. The ability of bacteria to divide by mitosis and thereby make clones of themselves. B.The double stranded DNA that your are amplifying with PCR … a. it functions at extremely high temperatures b. it adds nucleotide triphosphates to form a new DNA strand c. it requires a salt environment (buffer to work) d. It can act as an exonuclease to cut single-stranded DNA In order. Is note a true statements among the given statements. b. Both eukaryotic and prokaryotic DNA polymerases build off RNA primers made by primase. b. & 3. In restriction endonuclease EcoR1, “E” stands for. A) Replication of DNA begins with the uncoiling of the molecule. Get step-by-step explanations, verified by experts. It follows the law of complementary bases … 85) Each section of DNA that codes for a particular protein is called a ___. Which of the following is NOT true about PCR(polymerase chain reaction)? Question: Question 20 Which Of The Following Statements Is Not True? QUESTION 8 Which of the following would result in a mutation being inherited by both of the daughter cells after one round of cell division: O A mutation in Primase that increased its error rate O A single G-to-T transversion caused by cigarette smoke O Tautomerization of a single cytosine during DNA replication O Chemically-induced deamination of a single cytosine. 1) Terminal nucleotydyl transferease; 2) Ligase; 3) Alkaline Phosphatase; 4) DNA, b. 1) Ligase; 2) Polynucleotide kinase; 3) Alkaline Phosphatase; 4) DNA polymerase, c. 1) Ligase; 2) DNA polymerase; 3) Polynucleotide kinase; 4) Reverse transcriptase, d. 1) Ligase; 2) Polynucleotide kinase; 3) Restriction endonuclease 4) DNA, 23. E. coli DNA polymerase is capable of incorporating approximately 1000 nucleotides per second into a growing polynucleotide chain. | It is important for transcription c. It is important for translation d. It is important for PCR e. None of the above (all of the statements are true) Recombinant DNA: a. Introducing Textbook Solutions. © 2003-2020 Chegg Inc. All rights reserved. transcribe and translate these instructions are called ___. It is important for transcription c. It is important for translation d. It is important for PCR e. None of the above (all of the statements are true) Recombinant DNA: a. 79) Which of the following statements about the polymerase chain reaction are true? DNA (deoxyribonucleic acid) polymerase is an enzyme that catalyzes the formation of a phosphodiester bond. View desktop site, Question 7 Which of the following is NOTtrue of DNA polymerase: Solution: It uses ATP hydrolysis to fuel polymerization. If you have two DNA fragments A and B, both with blunt ends. 6. Terms O It must polymerize DNA 5' to 3'. It helps in replication to copy DNA using the single-stranded primer- either DNA or RNA. February, 2016. Almost all PCR applications employ a heat-stable DNA polymerase, such as Taq polymerase. to make it sticky which enzyme would you select for homopolymer tailing? Which of the following enzyme is suitable for dephosphorylation at 5’, 21. JavaScript is required to view textbook solutions. b) It polymerizes the short oligonucleotide strands formed during biosynthesis. A. DNA Polymerase Joins Nucleotides In One Direction (5' To 3') Only. B. by polymerase-catalyzed condensation of monomer nucleotides. e. DNA, Question 20 Which of the following statements is not true? (a) Micro-injection method (b) Gene gun method Which of the following enzyme is suitable for dephosphorylation at 5’ end of a DNA strand? This preview shows page 28 - 32 out of 46 pages. During DNA This preview shows page 10 - 14 out of 18 pages. b. © 2003-2020 Chegg Inc. All rights reserved. Which of the following statement is not true in case of DNA, 20. O It can only add nucleotides onto an existing polymer. Answer: adenine, cytosine, guanine, and uracil. Course Hero is not sponsored or endorsed by any college or university. The catalytic activity of eukaryotic DNA polymerase enzymes is approximately 2000 to 4000 nucleotides per minute. d. Generating an organism that is a genetic copy of another organism by bypassing sexual reproduce. c) a) anneal; b) denature the DNA sample; c) bind the monomer triphosphate nucleotide. a. DNA Polymerase b. dNTPs c. Helicase d. Primers e. None of the above (all are required) Which IS NOT true about complimentary base-pairing: a. Which statement about RNA polymerase is NOT true? nucleotides. For a limited time, find answers and explanations to over 1.2 million textbook exercises for FREE! d. Is required for human cloning. Answer: (d) All of the above. Large amounts of DNA must be isolated from the source organism. It adds dNTPs only in the ′ direction. B. Producing multiple copies of a recombinant DNA by transforming bacteria. University of Nevada, Las Vegas • CHEMISTRY 345, University of North Texas • CHEMISTRY 2380, Florida International University • CHM 2211. C. DNA Replication Proceeds In Only One Direction Around The Bacterial Chromosome D. Multiple Replication Forks Are Possible On A Bacterial Chromosome. The Lagging Strand Of DNA Is Started By An RNA Primer. d. Is required for human cloning. The Lagging Strand Of DNA Is Started By An RNA Primer. c. Is created from embryonic stem cells. double stranded copies will you obtain after 10 cycles? e. None of the above. Contains a segment of DNA from a foreign source. O It must polymerize DNA 5' to 3'. The mechanism by which the presence of arabinose (a sugar) controls the arabinose operon: a. catabolic repression b. induction c. translation d. anabolic repression fo. a. DNA polymerase joins nucleotides in one direction (5' to 3') only. e. All of the above, Which of the following IS NOT required for PCR?
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